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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
Ultrasensitive Enhanced Hrp Dab Situ Detection System, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for <t>SOX2,</t> NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).
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Image Search Results


Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for SOX2, NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).

Journal: STAR protocols

Article Title: Protocol for generating NGN2 iPSC lines and large-scale human neuron production.

doi: 10.1016/j.xpro.2025.103654

Figure Lengend Snippet: Figure 1. iPSC QC (A) Typical iPSC colony morphology without spontaneous differentiation. Colonies should have smooth edges, and minimal differentiated cells should be visible. Bright circular cells are floating dead cells (arrows), typically observed when cell density is high and does not affect culture quality. Scale Bar = 200 mm. (B) Example of qPCR karyotyping results. Chromosome 20 amplification was found in sample 15 (gray bars, red asterisk). Error bars: standard error of the mean. (C) Examples of KaryoStat Plus results. Top panel: sample with normal karyotype. Bottom panel: sample with chromosome 20 amplification (red arrow). (D) Example of immunofluorescence confirmation of pluripotency. Top: All cells (DAPI) are positive for SOX2, NANOG, and OCT4. Bottom: Patches of differentiated cells (white arrows) that are low on NANOG and OCT4. Scale Bars = 300 mm. (E) Example of ScoreCard results of iPSC and EB spontaneous differentiation samples. iPSCs should have minimal tri-lineage differentiation scores (blue and white), and EBs should have upregulated tri-lineage differentiation scores (red and pink).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies OCT3/4 (1:250) STEMCELL Technologies 60093.1 NANOG (1:250) R&D Systems AB_355097 SOX2 (1:250) Abcam AB_2341193 Donkey-anti-mouse-Alexa 647 (1:250) Jackson ImmunoResearch 715-605-151 Donkey-anti-goat-TRITC (1:250) Jackson ImmunoResearch 705-025-147 Donkey-anti-rabbit-Alexa 488 (1:250) Jackson ImmunoResearch AB_2313584 Chemicals, peptides, and recombinant proteins mTeSR Plus STEMCELL Technologies 100-0276 DMEM/F12 (no glutamine) Life Technologies 21331020 Neurobasal Life Technologies 21103049 CloneR2 STEMCELL Technologies 100-0691 Blasticidin S HCl (10 mg/mL) Gibco A1113903 Glutamax Gibco 35050061 NEAA Gibco 11140076 B27 without VA Gibco 12587010 N2 Max R&D Systems AR009 B27 Gibco 17504044 Y-27632 STEMCELL Technologies 72302 RevitaCell Gibco A2644501 Doxycycline R&D Systems 4090/50 DAPT STEMCELL Technologies 72082 Arabinocytidine Sigma C1768 Mitomycin C Tocris 3258/10 5-Fluorouracil Sigma F6627-1G SB431542 STEMCELL Technologies 72232 (Continued on next page) STAR Protocols 6, 103654, March 21, 2025 7 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Noggin Miltenyi 130-108-982 XAV939 STEMCELL Technologies 72672 BDNF Miltenyi 130-103-435 GDNF Miltenyi 130-129-546 Dibutyryl cyclic-AMP sodium salt (dcAMP) Sigma D0627 Ascorbic acid Sigma A4403 DPBS (without Ca2+ Mg2+) Gibco 14190144 iMatrix-511 Takara 892 012 Geltrex LDEV-Free, hESC-qualified, reduced growth factor Gibco A1413301 Poly-d-lysine (PDL) Sigma P6407-10X5MG ReLeSR STEMCELL Technologies 5872 Accutase STEMCELL Technologies 7920 7.5% BSA (tissue culture grade) Gibco 15260037 Papain Worthington LK003178 CryoStor CS10 STEMCELL Technologies 100-1061 Alt-R HDR Enhancer V2 IDT 10007921 Alt-R s.p.

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